After a certain delay, the bio-magnification of selenium continued in the higher trophic levels. This resulted in increased selenium concentrations and reduced methyl mercury concentrations in older perch and pike several years after the zooplankton, young roach and young perch. When the selenium treatments stopped the concentrations of selenium in the water, aquatic moss, bentic invertebrates and the youngest age groups of roach and perch fell immediately. The reductions in selenium in these parts of the ecosystem are related to the water retention period of the lake or reservoir.